Role of purinergic signaling in regulating IL-1α mediated inflammation inchronic kidney disease

MARYAM AMINI¹, PRISKA JOST¹, JANINA FRISCH², STEFAN SCHUNK³, JUTTA ENGEL², LETICIAPRATES ROMA², THIMOTEUS SPEER³, BARBARA A. NIEMEYER¹, and DALIA ALANSARY ¹

¹Molecular Biophysics, Saarland University, Homburg, Germany; ²Biophysics, Saarland University, Homburg, Germany; ³Department of Internal Medicine IV, Nephrology and Hypertension, Saarland University, Homburg, Germany

Inhibitory effect of Dexamethasone, a COVID-19 therapeutic on Kv1.3channels in CD8⁺ T lymphocytes

AMEET A. CHIMOTE¹, ABDULAZIZ ALSHWIMI¹, VAIBHAVKUMAR S. GAWALI¹, MARTINA CHIRRA¹, and LAURA CONFORTI ¹

¹Department of Internal Medicine, Division of Nephrology, University of Cincinnati, Cincinnati, Ohio

P2X4 and P2X7 are essential players in basal T cell activity and Ca²⁺ signalingmilliseconds after T cell activation

VALERIE J. BROCK¹, INSA M.A. WOLF¹, BJÖRN RISSIEK², MARCO ER-LUKOWIAK², FRIEDRICHKOCH- NOLTE³, TOBIAS STÄHLER³, CHRISTA E. MÜLLER⁴, LENA-MARIE WOELK⁵, RENÉWERNER⁵, ANDREAS H. GUSE¹, and BJÖRN-PHILIPP DIERCKS ^1*

¹The Ca²⁺ Signalling Group, Department of Biochemistry andMolecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany; ²Department of Neurology, University Medical Centre Hamburg Eppendorf, 20246 Hamburg, Germany; ³Department of Immunology, University Medical Centre Hamburg Eppendorf, 20246 Hamburg, Germany; ⁴Department of Pharmacy, University of Bonn, 53121 Bonn, Germany; ⁵Department of ComputationalNeuroscience, University Medical Centre Hamburg Eppendorf, 20246 Hamburg, Germany

NAADP forming enzyme in T cells

FENG GU¹, AILEEN KRÜGER¹, HANNES G. ROGGENKAMP¹, RICK ALPERS¹, DMITRI LODYGIN², VINCENT JAQUET³, FRANZISKA MÖCKL¹, LOLA C. HERNANDEZ C.¹, KAI WINTERBERG¹, ANDREAS BAUCHE¹, ANETTE ROSCHE¹, HELMUT GRASBERGER⁴, JOHN Y. KAO⁴, DANIELSCHETELIG⁵, RENÉ WERNER⁵, KATRIN SCHRÖDER⁶, MICHAEL CARTY⁷, ANDREW G BOWIE⁷, SAMUEL HUBER⁸, CHRIS MEIER⁹, HANS-WILLI MITTRÜCKER¹⁰, JOERG HEEREN¹, KARL-HEINZ KRAUSE³, ALEXANDER FLÜGEL², BJÖRN-PHILIPP DIERCKS¹, and ANDREAS H. GUSE ¹*

¹The Calcium Signaling Group, Dept. of Biochemistry and Molecular CellBiology, University Medical Center Hamburg-Eppendorf (UKE), 20246 Hamburg, Germany; ²Institute forNeuroimmunology and Multiple Sclerosis Research, University Medical Center Göttingen, 37075Göttingen, Germany; ³Dept. of Pathology and Immunology, University of Geneva, 1211 Geneva 4, Switzerland; ⁴Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109 USA; ⁵Dept. of Computational Neuroscience, UKE, Hamburg 20246, Germany; ⁶Institute of CardiovascularPhysiology, Goethe-Universität, 60590 Frankfurt, Germany; ⁷School of Biochemistry & Immunology, Trinity College Dublin, Dublin 2, Ireland; ⁸Dept. of Gastroenterology, UKE, 20246 Hamburg, Germany; ⁹Organic Chemistry, University of Hamburg, 20146 Hamburg, Germany; ¹⁰Dept. of Immunology, UKE, 20246 Hamburg, Germany

Adhesion-dependent T cell priming via FAK-PLC-γ pathway and SOCE activation demonstrated by advanced optical methods.

LOLA HERNANDEZ¹, MARIELLA KESSLER¹, ANKE LÖHNDORF¹, ANTONIO VIRGILIO FAILLA², ANDREAS H. GUSE¹, and BJÖRN-PH. DIERCKS¹

¹The Calcium Signaling Group, Dept. of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Germany, ²Microscopy Core Facility, University Medical CenterHamburg-Eppendorf, Germany

The voltage-gated potassium channel KV1.3 regulates neutrophil recruitmentduring inflammation

ROLAND IMMLER¹, WIEBKE NADOLNI², ANNIKA BERTSCH¹, VASILIOS MORIKIS³, INAROHWEDDER¹, SERGI MASGRAU-ALSINA¹, TOBIAS SCHROLL¹, ANNA YEVTUSHENKO¹, OLIVERSOEHNLEIN⁴, MARKUS MOSER⁵, THOMAS GUDERMANN², EYTAN R. BARNEA⁶, MARKUSREHBERG⁷, SCOTT I. SIMON³, SUSANNA ZIERLER², ⁸, MONIKA PRUENSTER¹ and MARKUS SPERANDIO ¹

¹Institute of CardiovascularPhysiology and Pathophysiology, Ludwig-Maximilians-UniversitätMünchen, 82152 Planegg-Martinsried, Germany, ²Walther-Straub Institute of Pharmacology and Toxicology, Ludwig-Maximilians-UniversitätMünchen, 80336 Munich, Germany, ³Department of Biomedical Engineering, Graduate Groupin Immunology, University ofCalifornia, Davis, CA 95616, USA, ⁴Center for Molecular Biology ofInflammation (ZMBE), Westfälische Wilhelms-UniversitätMünster, 48149 Münster, Germany, ⁵Institute ofExperimental Hematology, School of Medicine, Technical University Munich, 81675 Munich, Germany, ⁶BioIncept LLC, New York, NY 10016, USA, ⁷Institute of Lung Biology and Disease, Helmholtz ZentrumMünchen, 85764 Neuherberg, Germany, ⁸Institute of Pharmacology, Johannes Kepler University Linz, 4020 Linz, Austria

Chromogranin B anion channel and its potential roles in regulated secretion

GAYA P. YADAV^{1, #}, HAIYUAN WANG^{2, #}, JOKE OUWENDIJK³, MANI ANNAMALAI⁴, STEPHEN CROSS⁵, QIAOCHU WANG², D. WALKER HAGAN⁶, SUTONUKA BHAR¹, CLAYTON MATHEWS⁴, EDWARD A. PHELPS⁶, PAUL, VERKADE³, MICHAEL X. ZHU², and QIU-XING JIANG¹*

¹Department of Microbiology and Cell Science, University of Florida, 1355 Museum Drive, Gainesville, FL 32611-0700, USA; ²Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston, 6431Fannin St., Houston, TX 77030, USA; ³School of Biochemistry, Biomedical Sciences Building, UniversityWalk, University of Bristol, Bristol, BS8 1TD, UK; ⁴Department of Pathology, College of Medicine, University of Florida, 1275 Center Drive, Gainesville, FL 32611, USA; ⁵Wolfson Bioimaging facility, Biomedical Sciences Building, University Walk, University of Bristol, Bristol, BS8 1TD, UK; ⁶J. CraytonPruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA

Role of adhesion-dependent Ca²⁺ microdomains in T cell priming duringmigration to inflamed tissue

LOLA C. HERNANDEZ¹, DIANA C. GIL MONTOYA¹, ANKE LÖHNDORF¹, AILEEN KRÜGER¹, MIKOLAJNAWROCKI², SAMUEL HUBER², DANIEL SCHETELIG³, RENÉ WERNER³, ANTONIO V. FAILLA⁴, ALEXANDER FLÜGEL⁵, GENEVIÈVE DUPONT⁶, ANDREAS H. GUSE¹, BJÖRN-PHILIPP DIERCKS¹, and MARIELLA KESSLER ¹

¹The Ca²⁺ Signalling Group, Department of Biochemistry and Molecular Cell Biology, University MedicalCenter Hamburg-Eppendorf, 20246 Hamburg, Germany; ²Section of Molecular Immunology undGastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg20246, Germany; ³Department of Computational Neuroscience, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany; ⁴Microscopy Imaging Facility (UMIF), University Medical CenterHamburg-Eppendorf, Hamburg, Germany; ⁵Institute for Neuroimmunology and Multiple SclerosisResearch, University Medical Centre Göttingen, 37075 Göttingen, Germany; ⁶Unité de ChronobiologieThéorique, Faculté des Sciences, CP231, Université Libre de Bruxelles, B-1050 Brussels, Belgium

HN1L/JPT2: a signaling protein connecting NAADP to Ca²⁺ microdomains

IMRANKHAN KHANSAHIB¹, HANNES G. ROGGENKAMP¹, LOLA C. HERNANDEZ¹, YUNPENGZHANG¹, DMITRI LODYGIN², AILEEN KRÜGER¹, FENG GU¹, FRANZISKA MÖCKL¹, ANKELÖHNDORF¹, VALERIE WOLTERS¹, DANIEL WOIKE¹, ANETTE ROSCHE¹, ANDREAS BAUCHE¹, DANIEL SCHETELIG³, RENÉ WERNER³, HARTMUT SCHLÜTER⁴, ANTONIO V. FAILLA⁵, RALFFLIEGERT¹, CHRIS MEIER⁶, TIMOTHY F. WALSETH⁷, ALEXANDER FLÜGEL², BJÖRN-PHILIPP DIERCKS¹*, and ANDREAS H. GUSE

¹The Ca²⁺ Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Germany; ²Institute of Neuroimmunology, Universitätsmedizin Göttingen, Germany; ³Department of Computational Neuroscience, University Medical Center Hamburg-Eppendorf, Germany; ⁴MassSpectrometric Proteomics Group, Institute of Clinical Chemistry and Laboratory Medicine, University Medical Center Hamburg- Eppendorf, Germany; ⁵Microscopy Imaging Facility (UMIF), UniversityMedical Center Hamburg-Eppendorf, Germany; ⁶Institute of Organic Chemistry, University of Hamburg, Germany; ⁷Dept. of Pharmacology, University of Minnesota, Minneapolis, USA

The ion channel TRPV5 regulates B cell activation and signalling

TRISHA MAHTANI¹, LOGAN K. SMITH², LESHAWN BENEDICT² and BEBHINN TREANOR ^{1, 2, 3}

¹Department of Cell and Systems Biology, University of Toronto, ON, Canada; ²Department of Biological Sciences, University of Toronto Scarborough, ON, Canada; ³Department of Immunology, University of Toronto, ON, Canada

Thermosensitive TRP channels modulate T cell activation

RAKESH KUMAR MAJHI* and CHANDAN GOSWAMI ^#

School of Biological Sciences, National Instituteof Science Education and Research, Bhubaneswar, India; correspondence: majhi.rakesh@gmail.com, chandan@niser.ac.in

Inositol 1, 4, 5-trisphosphate 3-kinase B promotes Ca²⁺ mobilization and theinflammatory activity of dendritic cells

LAURA MARONGIU^{1, 6}, FRANCESCA MINGOZZI¹, MASSIMILIANO GARRȲ, DARIO PARAZZOLI², LAURA SIRONI³, REIKO SAKAGUCHI4, TAKASHI MORII⁵, MARIACRISTINA CROSTI⁶, MONICAMORO⁶, STÉPHANE SCHURMANS7, TIZIANO CATELANI⁸, RANY ROTEM¹, MIRIAM COLOMBO¹, STEPHEN SHEARS⁹, DAVIDE PROSPERI¹, IVAN ZANONI¹⁰, and FRANCESCA GRANUCCI ^{1, 6}

¹Department of Biotechnology andBiosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy; Division ofGastroenterology, Boston Children’ s Hospital, Boston, MA 02115, USA; ²IFOM, FIRC Institute of MolecularOncology, Milan, Italy; ³Department of Physics, University of Milano-Bicocca, Piazza della Scienza 3, 20126 Milan, Italy; ⁴Institute for Integrated Cell-Material Sciences, Kyoto University Katsura, Nishikyo-ku, Kyoto 615-8510, Japan; ⁵Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan; ⁶INGM, Istituto Nazionale di Genetica Molecolare “ Romeo ed Enrica Invernizzi” , 20122 Milan, Italy; ⁷Laboratory of Functional Genetics, GIGA-B34, University of Liège, 4000 Liège, Belgium; ⁸Piattaforma Interdipartimentale di Microscopia, University of Milano-Bicocca, Piazza della Scienza 3, 20126 Milan, Italy; ⁹Signal Transduction Laboratory, NIEHS/NIH, 111 TW Alexander Drive, ResearchTriangle Park, NC 27709, USA; ¹⁰Division of Immunology, Harvard Medical School, Boston Children' sHospital, Boston, MA 02115, USA

Cryo-EM of channels and transporters: What have we learned in the lastdecade and what are the current challenges?

DOREEN MATTHIES

Unit on Structural Biology, Division of Basic and Translational Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes ofHealth, Bethesda, MD, USA

A succinate transport pathway delivers succinate to macrophages thus

perpetuating their pro-inflammatory metabolic state

MORAN FREMDER, SEUNG WON KIM, AHLAM KHAMAYSI, LIANA SHIMSHILASHVILI, HADAR EINI-RIDER, ISEUL PARK, UZI HADAD, JAE HEE CHEON, and EHUD OHANA

Ben Gurion University of the Negev, Beer Sheva, Israel

Method of measuring the membrane potential of phagosomes by opticalimaging

YOSHIFUMI OKOCHI¹, HIDEKAZU TSUTSUI² and YASUSHI OKAMURA ¹

¹Integrative physiology, Graduate school of medicine, Osaka university; ²Japan Advanced Institute of Science and Technology(JAIST)

Neutrophil extracellular trap (NET) formation in mice with Stim1/2 myeloid-specific ablation.

CAMILLE RABESAHALA DE MERITENS, AMADO CARRERAS-SUREDA, and NICOLAS DEMAUREX

Department of Cellular Physiology and Metabolism, University of Geneva, Switzerland

Bioengineered bacteria for the oral delivery of Kv1.3 blockers for thetreatment of autoimmune diseases

YUQING WANG¹, DUOLONG ZHU², LAURA C, ORTIZ-VELEZ², J. LANCE PERRY², MICHAEL W.PENNINGTON³, JOSEPH M, HYSER^{1, 2}, ROBERT A, BRITTON², and CHRISTINE BEETON ¹

¹Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX; ²Department of MolecularVirology and Microbiology and Alkek Center for Metagenomics and Microbiome Research, Baylor Collegeof Medicine, Houston, TX; ³AmbioPharm Inc., North Augusta, SC, US

NADPH oxidases produce NAADP under physiological conditions

KAI WINTERBERG¹, RICK ALPERS¹, FENG GU¹, HANNES G. ROGGENKAMP¹, AILEEN KRÜGER¹, DMITRI LODYGIN², VINCENT JAQUET³, FRANZISKA MÖCKL¹, LOLA C. HERNANDEZ¹, ANDREAS BAUCHE¹, ANETTE ROSCHE¹, HELMUT GRASBERGER⁴, JOHN Y. KAO⁴, DANIEL SCHETELIG⁵, RENÉ WERNER⁵, KATRIN SCHRÖDER⁶, MICHAEL CARTY⁷, ANDREW G. BOWIE⁷, SAMUELHUBER⁸, CHRIS MEIER⁹, HANS-WILLI MITTRÜCKER¹⁰, JOERG HEEREN¹, KARL-HEINZ KRAUSE³, ALEXANDER FLÜGEL², BJÖRN-PHILIPP DIERCKS¹, and ANDREAS H. GUSE ¹

¹The Calcium Signaling Group, Dept. of Biochemistry and Molecular CellBiology, University Medical Center Hamburg-Eppendorf (UKE), 20246 Hamburg, Germany, ²Institute for Neuroimmunology and Multiple Sclerosis Research, University Medical Center Göttingen, 37075 Göttingen, Germany, ³Dept. of Pathology and Immunology, University of Geneva, 1211 Geneva 4, Switzerland, ⁴Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109 USA, ⁵Dept. ofComputational Neuroscience, UKE, Hamburg 20246, Germany, ⁶Institute of Cardiovascular Physiology, Goethe-Universität, 60590 Frankfurt, Germany, ⁷School of Biochemistry & Immunology, Trinity College Dublin, Dublin 2, Ireland, ⁸Dept. of Gastroenterology, UKE, 20246 Hamburg, Germany, ⁹Organic Chemistry, University of Hamburg, 20146 Hamburg, Germany, ¹⁰Dept. of Immunology, UKE, 20246 Hamburg, Germany

Extracellular histone proteins activate P2X7 channels

MAXWELL S. ROSS, RUA’ A AL-AQTASH, and DANIEL M. COLLIER

UTHSC College of Pharmacy, Dept. of Pharmaceutical Sciences, Memphis, TN 38163

Goblet cell LRRC26 regulates BK channel activation and protects againstcolitis in mice

VIVIAN GONZALEZ-PEREZ¹, PEDRO L. MARTINEZ-ESPINOSA¹, MONICA SALA-RABANAL¹, NIKHILBHARADWAJ¹, XIAO-MING XIA¹, ALBERT C. CHEN^{1, 5}, DAVID ALVARADO², JENNY K.GUSTAFSSON^{2, 3, 4}, HONGZHEN HU¹, MATTHEW A. CIORBA², and CHRISTOPHER J. LINGLE ¹

¹Department ofAnesthesiology, Washington University School of Medicine, St. Louis, MO, USA, 63110; ²Department ofInternal Medicine, Division of Gastroenterology, Washington University School of Medicine, St. Louis MO, USA, 63110; ³Department of Medical Chemistry and Cell Biology and ⁴Department of Physiology, University of Gothenburg, Gothenburg, Sweden; ⁵McKelvey School of Engineering at WashingtonUniversity in St. Louis

The role of immune cells in dermal white adipose tissue homeostasis

EDRIES YOUSAF HAJAM and COLIN JAMORA

Centre for Inflammation and Tissue Homeostasis, Institute for Stem Cell Science and Regenerative Medicine

Mechanism of glucocorticoid-induced TNF receptor activation by an agonisticantibody

CHANGHAO HE^{1, #}, RACHANA R. MANIYAR^{2, #}, YAHEL AVRAHAM⁶, ROBERTA ZAPPASODI^{2, 3, 4, †} , RADDA RUSINOVA¹, WALTER NEWMAN⁷, HEIDI HEATH⁷, JEDD D. WOLCHOK^{2, 3, 4, 5}, RONY DAHAN⁶, TAHA MERGHOUB^{2, 3, 4, 5, *}, and JOEL R. MEYERSON ^1*

¹Department of Physiology and Biophysics, WeillCornell Medical College, New York, NY, USA, ²Ludwig Collaborative and Swim Across America Laboratory, Human Oncology and Pathogenesis Program, MSK, New York, NY, USA, ³Parker Institute for CancerImmunotherapy, MSK, New York, NY, USA, ⁴Weill Cornell Medicine, New York, NY, USA, ⁵Department ofMedicine, MSK, New York, NY, USA, ⁶Department of Immunology, Weizmann Institute of Science, Rehovot, Israel, ⁷Leap Therapeutics, Cambridge, MA, USA

The function of TRPM7 channel-kinase in T lymphocytes and macrophages:insights from transgenic mouse models

J. ASHOT KOZAK

Department of Neuroscience, Cell Biology and Physiology, Boonshoft School ofMedicine, Wright State University, Dayton, OH 45435

Engineering of photoswitchable single-domain intrabodies for biomedicalapplications

YI-TSANG LEE¹, LIAN HE¹, PENG TAN³, YUN HUANG², and YUBIN ZHOU¹

¹Institute of Biosciencesand Technology Texas A& M University, Center for Translational Cancer Research, Houston, TX; ²Institute of Biosciences and Technology Texas A& M University, Center for Epigenetics and Disease Prevention, Houston, TX; ³Broad Institute of MIT and Harvard, Cambridge, MA

Identification of a STIM1 splicing variant that promotes glioblastoma cell growth

GUOLIN MA and YUBIN ZHOU *

Center for Translational Cancer Research, Institute of Biosciences andTechnology, Texas A& M University, Houston, TX, 77030, USA. +1-713-677-7483, gma@tamu.edu; yubinzhou@@tamu.edu

Inflammatory IL-1β release from Gasdermin D pores is dynamically regulated by calcium-phosphoinositide signaling

GARY CH MO

Department of Pharmacology and Regenerative Medicine, University of Illinois at Chicago, Chicago, USA

Cm28, a peptide from the venom of Centruroides margaritatus, obeys unique primary structure and inhibits Kv1.2 and Kv1.3 with high affinity

MUHAMMAD UMAIR NASEEM¹, JOSÉ BELTRÁN-VIDAL^{2, 3}, EDSON CARCAMO-NORIEGA⁴, LOURIVAL D. POSSANI⁴, and GYORGY PANYI ¹*

¹University of Debrecen, Faculty of Medicine, Department ofBiophysics and Cell Biology, Egyetem ter 1, Life Science Bldg. 2.305, Debrecen, 4032, Hungary; ²Grupo de Investigaciones Herpetológicas y Toxinológicas, Centro de Investigaciones Biomédicas, Departamento de Biología, Facultad de Ciencias Naturales, Exactas y de la Educación, Universidad del Cauca, SectorTulcan, Calle 2 N 3N-100, Popayán 190002, Cauca, Colombia; ³Grupo de Investigación Laboratorio deHerpetología y Toxinología, Departamento de Fisiología, Facultad de Salud, Universidad del Valle, Calle4B N° 36-00, Santiago de Cali 760043, Valle del Cauca, Colombia; ⁴Departamento de Medicina Moleculary Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Av. Universidad2001, Cuernavaca 62210, Morelos, Mexico; *corresponding author: panyi@med.unideb.hu

Role of ERG1 K⁺ channel in lymphocyte development

CESARE SALA and ANNAROSA ARCANGELI

Department of Experimental and Clinical Medicine, University of Florence, Viale Morgagni 50, Florence, Italy, Cesare.sala@unifi.it +39 0552751231, Annarosa.arcangeli@unifi.it +39 0552751285

Blockers of TRPM7 & TRPM6 cation channels inhibit proliferation of B-lymphocyte lymphoma/leukemia cancers

MARK S. SHAPIRO, PH.D., AIOLA STOJA, NINA BOIKO, PH.D., and JAMES STOCKAND, PH.D.

UTHSCSA

shapirom@uthscsa.edu, 8403 Floyd Curl Drive, San Antonio, TX 78229, 210-562-4092; UTHSCSA, stoja@uthscsa.edu, 8403 Floyd Curl Drive, San Antonio, TX 78229, 210-562-4075; UTHSCSA, boiko@uthscsa.edu, 7703 Floyd Curl Drive, San Antonio, TX 78229, 210-567-4360; UTHSCSA, stockand@uthscsa.edu, 7703 Floyd Curl Drive, San Antonio, TX 78229, 210 567 4332

Pharmacologic inhibition of TPC1 diminishes structural basis for endoplasmicreticulum and endo-lysosome interactions

PHILIP STEINER¹, ANCUELA ANDOSCH², KARIN OBERASCHER², THOMAS GUDERMANN³, INGRID BOEKHOFF³, HUBERT KERSCHBAUM, ² and SUSANNA ZIERLER ^{1, 3}

¹Institute of Pharmacology, Medical Faculty, Johannes Kepler University, Linz, Austria; ²Department of Biosciences, Paris LodronUniversity, Salzburg, Austria; ³Walther Straub Institute of Pharmacology and Toxicology, Faculty ofMedicine, Ludwig-Maximilians University Munich, Germany

Understanding the voltage-gated proton channel (Hv1) pH dependence togain insights into its function in the immune system

EMERSON M. CARMONA^{1, 2}, MIGUEL FERNANDEZ¹, ALAN NEELY¹, H. PETER LARSSON³, OSVALDO ALVAREZ^{1, 4}, JOSE A. GARATE¹, RAMON LATORRE¹, LUIS G. CUELLO², and CARLOSGONZALEZ ¹

¹Centro Interdisciplinario de Neurociencia de Valparaiso, Universidad de Valparaiso, Valparaiso, Chile; ²CellularPhysiology and Molecular Biophysics, Texas Tech University Health Sciences Center, Lubbock, TX, USA; ³Department of Physiology and Biophysics, Miller School of Medicine, University of Miami, Miami, FL, USA; ⁴Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile

The volume regulated anion channel LRRC8C suppresses T cell function byregulating cyclic dinucleotide transport and STING-p53 signaling

AXEL R. CONCEPCION, ¹ LARRY E. WAGNER II, ² JINGJIE ZHU, ¹ ANTHONY Y. TAO, ¹ JUN YANG, ¹ALIREZA KHODADADI-JAMAYRAN, ³ YIN-HU WANG, ¹ MENGHAN LIU, ¹ WILLIAM A. COETZEE, ¹DAVID I. YULE, ² and STEFAN FESKE ¹

¹Department of Pathology, New York University School of Medicine, New York, NY10016, USA; ²Department of Pharmacology and Physiology, University of Rochester, 601 Elmwood Ave, Rochester. NY 14642, USA; ³Applied Bioinformatics Laboratories, NYU School of Medicine, New York, NY10016, USA

Deletion of myeloid Pannexin-1 channels decrease chemokines expressionand improves cognitive function after brain trauma

MILONI S. DALAL¹, JOON S. HEO², and JORGE E. CONTRERAS ^{1, 3}

¹Department of Pharmacology, Physiology and Neuroscience, New Jersey Medical School, Rutgers University, Newark, NJ 07103, USA; ²Department of Neurology and Nash Family, Department of Neuroscience, Friedman BrainInstitute, Icahn School of Medicine, Mount Sinai, New York, NY 10029, USA; ³Department of Physiology and Membrane Biology, University of California Davis, Davis, CA 95616, USA

Functional reconstitution of a mitochondrial calcium uniporter

BRYCE D. DELGADO^{1, 2} and STEPHEN B. LONG ¹

¹Structural Biology Program, Memorial SloanKettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA; ²Graduate Program inBiochemistry and Structural Biology, Cell and Developmental Biology, and Molecular Biology, Weill CornellMedicine Graduate School of Medical Sciences, New York, USA

Cavb1 regulates T cell expansion and apoptosis independently of voltage-gated Ca²⁺ channel function

SERAP ERDOGMUS^1*, AXEL R. CONCEPCION^1*, IKJOT SIDHU¹, ANTHONY TAO¹, WENYI LI¹, PEDRO P. ROCHA^{2, 3}, BONNIE HUANG^{4, 5}, RALPH GARIPPA⁶, BORAM LEE⁷, AMY LEE⁸, JOHANNESW. HELL⁷, RICHARDS. LEWIS⁹, MURALI PRAKRIYA¹⁰, and STEFAN FESKE ¹

¹Department of Pathology, NYU GrossmanSchool of Medicine, New York, NY, USA; ²Unit on Genome Structure and Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA; ³NationalCancer Institute, NIH, Bethesda, MD, USA; ⁴National Institute of Allergy and Infectious Disease; Bethesda, MD, USA; ⁵National Human Genome Research Institute, Bethesda, MD, USA; ⁶Memorial Sloan KetteringCancer Center, Department of Cancer Biology & Genetics, New York, NY, USA; ⁷Department ofPharmacology, University of California, Davis, CA, USA; ⁸Department of Neuroscience, University ofTexas-Austin, TX, USA; ⁹Department of Molecular and Cellular Physiology, Stanford University, Palo Alto, CA; ¹⁰Department of Pharmacology, Northwestern University, Chicago, IL; *These authors contributedequally

Orai Channel C-terminal Peptides – Calcium Signal Suppression and Potentialfor Immunomodulation

JAMES H. BARANIAK, JR., YANDONG ZHOU, and DONALD L. GILL

Department of Cellular andMolecular Physiology, Penn State College of Medicine, Hershey PA 17033

Gating and conduction in the KcsA and Kv1.2 channels from quantumcalculations

ALISHER M. KARIEV and MICHAEL E GREEN

Department of Chemistry and Biochemistry, CCNY, 160Convent Ave, New York, NY 10031, akariev@ccny.cuny.edu and mgreen@ccny.cuny.edu

Novel compounds target drug-resistant Neuroblastoma through mechanisminvolving store-operated calcium entry

INGO LANGE, ESPINOZA-FUENZALIDA, NATHAN SUNADA, LI FENG, DIANQING SUN, and DANA-LYNN T. KOOMOA

The University of Hawaii at Hilo, The Daniel K. Inouye College of Pharmacy

Distinct roles of ORAI1 in T cell mediated allergic airway inflammation andantiviral immunity in the lung

SASCHA KAHLFUSS^{1, 3}, YIN-HU WANG¹, LUCILE NOYER¹, SEAN P SAUNDERS², DIMITRIUSRAPHAEL¹, ANTHONY TAO¹, ULRIKE KAUFMANN^{1, 4}, MARISA MITCHELL-FLACK¹, IKJOT SIDHU¹, MARTIN VAETH^{1, 5}, PAUL G. THOMAS⁶, MARIA A. CUROTTO DE LAFAILLE², and STEFAN FESKE ¹

¹Department of Pathology, New York University Grossman School of Medicine, New York, NY, USA; ²Division of Pulmonary, CriticalCare and Sleep Medicine, Departments of Medicine and Cell Biology, New York University GrossmanSchool of Medicine, NY, 10016, USA; ³Current address: Medical Faculty, Otto-von-Guericke-Universität, Magdeburg, Germany; ⁴Current address: Genentech, South San Francisco, CA, USA; ⁵Currentaddress: Max Planck Research group for Systems Immunology, Julius Maximilians University, Wuerzburg, Germany; ⁶St. Jude' s Children' s Research Hospital, Memphis, TN, USA

STIM2 in colorectal cancer metabolism and progression

TRAYAMBAK PATHAK and MOHAMED TREBAK

Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania

TRPM7 inactivation and the role of pH in phagocytic activity of murinemacrophages

JANANIE ROCKWOOD*, PAVANI BEESETTY*, TETYANA ZHELAY*, SIHAM HOURANI*, TAKU KAITSUKA**, MASAYUKI MATSUSHITA^# and J. ASHOT KOZAK *

*Department of Neuroscience, Cell Biology andPhysiology, Boonshoft School of Medicine, Wright State University, Dayton, OH 45435, USA; **School ofPharmacy at Fukuoka, International University of Health & Welfare, Fukuoka, Japan; ^#Department ofMolecular and Cellular Physiology, University of the Ryukyus, Okinawa, Japan

Suppression of Ca2+ signals by EGR4 controls Th1 differentiation and anti-cancerimmunity in vivo

JAYATI MOOKERJEE-BASU^1*, ROBERT HOOPER^{2, 3*}, SCOTT GROSS^{2, 3}, BRYANT SCHULTZ^{2, 3}, CHRISTINA K. GO^{2, 3}, ELSIE SAMAKAI^{2, 3}, JONATHAN LADNER¹, EMMANUELLE NICOLAS¹, YUANYUAN TIAN^{2, 4}, BO ZHOU², M. RAZA ZAIDI^{2, 3}, WARREN TOURTELLOTTE⁵, SHAN HE^{2, 4}, YI ZHANG^{2, 4}, DIETMAR J KAPPES¹, and JONATHAN SOBOLOFF^{2, 3}

¹Fox Chase Cancer Center, Philadelphia, PA 19111, USA; ²FelsInstitute for Cancer Research and Molecular Biology & Departments of ³Medical Genetics & MolecularBiochemistry and ⁴Immunology, Temple University School of Medicine, Philadelphia, PA, 19140, USA; ⁵Cedars Sinai Medical Center, Department of Pathology and Laboratory Medicine, West Hollywood, CA90048

ORAI1 establishes resistance to SARS-CoV-2 infection by regulating tonictype I interferon signaling

BEIBEI WU, ARUNACHALAM RAMAIAH, GUSTAVO GARCIA JR., YOUSANG GWACK, VAITHILINGARAJA ARUMUGASWAMI, and SONAL SRIKANTH

Beibei Wu, Yousang Gwack and Sonal Srikanth, Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA, beibeiwu@mednet.ucla.edu, ygwack@mednet.ucla.edu, ssrikanth@mednet.ucla.edu; Arunachalam Ramaiah, Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA, arunachalamphd@gmail.com; Gustavo Garcia Jr., Vaithilingaraja Arumugaswami, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA, GustavoGarcia@mednet.ucla.edu, VArumugaswami@mednet.ucla.edu

Targeting the ion channel TRPM7 promotes the thymic development ofregulatory T cells by promoting IL-2 signaling

MARTA E. STREMSKA^{1, 2, *}, SURESH K. MENDU^1, *, MICHAEL S. SCHAPPE³, EMILY K. MOSER⁴, JULIA K. KRUPA¹, JASON S. ROGERS¹, ERIC J. STIPES¹, CLARE A. PARKER¹, THOMAS J.BRACIALE², JUSTIN S. A. PERRY^{6, 7, 8}, AND BIMAL N. DESAI¹

^*Equal authors, ¹Department of Pharmacology and ²Department ofMicrobiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, VA 22908, USA, ³Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Boston, MA02115, USA, ⁴University of Florida, Gainesville, FL 32610, USA.⁶Immunology Program, Memorial SloanKettering Cancer Center, New York, NY 10065, USA, ⁷Louis V. Gerstner Jr. Graduate School ofBiomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA, ⁸Department ofImmunology and Microbial Pathogenesis, Weill Cornell Medical College, New York, NY 10065, USA

Transcriptomic and forward genetic screens to elucidate the role of ion channelsand transporters in B cells

ANTHONY TAO and STEFAN FESKE

New York University Grossman School of Medicine, New York, NY10016

Investigation of novel ion channels as potential next-generation therapeutictargets for MS

LIWEI WANG, AXEL CONCEPCION, IKJOT SIDHU, ANTHONY TAO, ULRIKE KAUFMANN, and STEFAN FESKE

Department of Pathology, New York University School of Medicine, New York, NY 10016, USA

Characterization of CRAC and other ion channels in inflammatory bowel disease

YIN-HU WANG¹, MARILENA LETIZIA^{2, 3}, ULRIKE KAUFMANN¹, CARL WEIDINGER^{2, 3}, and STEFAN FESKE ¹

¹Department of Pathology, New York University Grossman School of Medicine, New York, NY, USA; ²Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health; ³Department of Gastroenterology, Infectious Diseasesand Rheumatology, Campus Benjamin Franklin

A tale of yin and yang: TMEM16F gating, ion selectivity, and functional implications

WENLEI YE, TINA W. HAN, MU HE, YUH NUNG JAN, and LILY Y. JAN

University of California, SanFrancisco, United States; Howard Hughes Medical Institute, United States

PIEZO1 mediates a novel mechano-thrombotic pathway in diabetes

WANDI ZHU^{1, 2}, SHIHUI GUO³, MAX HOMILIUS^{1, 2}, CISSY NSUBUGA¹, SHANE H. WRIGHT^{1, 4}, DAJUNQUAN¹, MANU BEERENS^{1, 2}, ROBERT FLAUMENHAFT^{2, 3}, RAHUL C. DEO^{1, 2}, and CALUM A.MACRAE ^{1, 2}*

¹Department of Medicine, Brigham and Women' s Hospital, Boston, Massachusetts, United States; ²Harvard Medical School, Boston, Massachusetts, United States; ³Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States; ⁴Department of BiomedicalEngineering, Boston University, Boston, Massachusetts, United States